On the contrary, IGF1R inhibitor treatment effectively reversed the activation effect of miR-4521 knockdown on IGF1R/AKT/GSK3β/Snai1 pathway, suggesting that inactivation of IGF2 interferes with the negative regulation of the AKT/GSK3β/Snai1 pathway by miR-4521 in GC cells. The gene discussed is IGF2; the disease is gastric cancer.