To investigate whether the combined inhibition of PI3Kβ and MLK3 could synergistically inhibit GBM cell migration and invasion, U-87 MG cells were treated with 24 μM of AZD6482 and 4 μM of URMC-099, while U-118 MG cells were treated with 30 μM of AZD6482 and 3 μM of URMC-099, since cells treated with inhibitors at these concentrations showed synergistic effect but less cytotoxicity. Here, MAP3K11 is linked to glioblastoma.