Hence our findings conclude that GABA mimetic effects of POL-6 normalizes Gabra1, Gabra2, Gabra3, Gabra4, and Gabra5 genes of GABAA receptor subunits in rats amygdala and hippocampus and showed protective effects during ethanol withdrawal anxiety especially at higher concentrations 50 and 100 mg/kg/b.wt. Here, GABRA3 is linked to Anxiety.