Univariate analysis showed that age, preoperative hemoglobin (Hb), preoperative cystatin C (Cys-C), operation duration, CPB time, aortic cross-clamp (ACC) time, number of valve replacements, peak TB concentration, and time to peak TB concentration were significantly associated with AKI after HVS. The gene discussed is GSTM1; the disease is acute kidney injury.