For this, we predicted the top ten candidate reference genes (ACTB, RPS27, RPS11, RPL13A, RPL41, RPS14, RPL41P1, RPS29, RPL10 and NACA), in cancer tissues with simultaneous high expression abundance (log2(TPM + 1)) and stable expression (“CV” and “M”) (Suppl. The gene discussed is RPS27; the disease is cancer.