As demonstrated (Figure 7, red dots), the best concentration sensitivity at the level for proteins concentrated on the surface of the AFM chip using affinity methods was achieved for objects characterized by different molecular weights from 20 to 120 kDa and an aliphatic index value from 75 to 91—glycoprotein HIV-1 gp120 (LOD 10−11 M and TOF), human serum albumin (HSA, LOD 10−9 M, IT, and TOF), bovine serum albumin (BSA, LOD 10−10 M, and TOF), core antigen of viral hepatitis C (HCVcoreAg, LOD 10−13 M, and TOF), horseradish peroxidase (HRP, LOD 10−15 M, and QqQ). Here, ALB is linked to hepatitis C virus infection.