PRF1 and neoplasm: Indeed, overexpression of GSDME in cancer cells significantly increased the number of intra-tumoral NK cells and antigen-specific CD8+ T, the expression of granzyme B, perforin, IFN-γ, and TNF-α in tumor-infiltrating lymphocytes (TILs), and phagocytosis of tumor cells by tumor-associated macrophages.