The analysis showed that miRNAs downregulated both by the hotspot and deleterious SMAD4 mutations are associated (adjusted P < 0.01) with similar cancer-related processes, including ‘Pathways in cancer’, ‘ErbB signaling pathway’, ‘Glioma’, and ‘Proteoglycans in cancer’ (Supplementary Table S6). Here, EGFR is linked to cancer.