In the current study, based on the myostatin‐mediated PI3K‐Akt‐FoxO3a pathway and the proliferation and differentiation function of satellite cells, we investigated the role and mechanisms of FMN in CKD muscle atrophy in 5/6 nephrectomy rats in vivo and a TNF‐α‐induced C2C12 in vitro myotubes model. Here, FOXO3 is linked to chronic kidney disease.