ALB and nutritional deficiency disease: 2013). Decreased total protein concentration can also be caused by malnutrition and mal-absorption, liver disease, chronic and acute diarrhoea, burning, hormonal imbalance, kidney disease (proteinuria), low albumin concentration, and low globulin concentration (Meiklejohn et al. 2019). An increase in total bilirubin levels and direct bilirubin activity and a decrease in albumin levels and total protein level in the hepatotoxic-induced rat is indicating damage to the integrity or function of liver cells due to paracetamol intoxication (Valášková and Muchová 2016; Yoon et al. 2016).