In univariate analysis, we found that histological differentiation grade (p = 0.003), depth of invasion (p = 0.016), lymph node metastasis (p = 0.002), TNM stages (p = 0.011), nuclear HIF-1A expression (p< 0.001) were statistically significant factors for OS (Table 7). The gene discussed is HIF1A; the disease is metastatic malignant neoplasm in the lymph nodes.