Several independent studies reported that increased HIF-1A expression was associated with poor prognosis in many cancers 16, 46, but the prognostic value of HIF-1A expression in GBC is still controversial 22-24, however, we did not study the relationship between cytoplasmic HIF-1A expression and nuclear HIF-1A expression and GBC prognosis separately. This evidence concerns the gene HIF1A and cancer.