In the present study, we found that tumors located in the upper respiratory tract (larynx) showed significantly higher FOXD1 expression levels than those in the upper gastrointestinal tract (oral cavity, oropharynx and hypopharynx), which suggested that laryngeal squamous cell carcinoma may be more susceptible to FOXD1 overexpression. This evidence concerns the gene FOXD1 and laryngeal squamous cell carcinoma.