Although levels of the representative activation markers for microglia (AIF1, CD68, and LGALS3) were unchanged, expressions of BCO2, CAPN3, small G protein-associated genes (RASGRP3 and RAPGEF5), PACC1 (also known as TMEM206), and P2RX7 were significantly decreased in AD precuneus compared with controls (Fig. 4c). This evidence concerns the gene RAPGEF5 and Alzheimer disease.