Comparison of AD risk genes among the three groups (human AD, AppNL-G-F/NL-G-Fand rTg4510) revealed that 12 genes (Apoe, Scimp, Pld3, Psen1, Psen2, Sqstm1, Trem2, Treml2, Ap2a2, Cass4, Lmo4 and Hbegf) were commonly altered in AppNL-G-F/NL-G-Fand rTg4510 cortical microglia, and only one gene (HBEGF/Hbegf) was significantly decreased in AD precuneus as well as AppNL-G-F/NL-G-Fand rTg4510 cortical microglia (Fig. 6b and Additional file 1: Table S1i). This evidence concerns the gene PSEN2 and Alzheimer disease.