To further study correlations between NGF and CHRM4 in human prostate tissues, we analyzed 16 normal prostatic epithelial samples, 81 primary low-grade prostate adenocarcinomas, 19 primary high-grade prostate adenocarcinomas, and 14 SCNCs from a prostate tissue microarray (TMA) collected from the Department of Pathology at Duke University School of Medicine (Durham, NC, USA). This evidence concerns the gene NGF and prostate adenocarcinoma.