We focused on characterizing the effect of PIM1-dependent serine 330 phosphorylation (pS330) of NDRG1, given that a phosphoproteomic analyses of prostate cancer tissues by Drake et al.43 demonstrated that levels of NDRG1 pS330 were found to be 7.7 times higher in metastatic lesions than in localized prostate cancer (Supplementary Data 1). Here, PIM1 is linked to prostate cancer.