Without etoposide treatment, the percentage of aneuploidy in KIFC1-WT and KIFC1-S26A cells was low and similar to that in untreated MCF-10A cells, indicating that etoposide-induced centrosome amplification and clustering were necessary for the occurrence of CIN (Fig. 7e, f). The gene discussed is KIFC1; the disease is cervical squamous intraepithelial neoplasia.