The scRNA-seq data of the human alveolar rhabdomyosarcoma cell line Rh41 were downloaded from Gene Expression Omnibus (GEO) (GSE113660) to validate the distribution and expression of key genes (PAX5, LTB, IL1A, IL5RA, IL7, LY9, SLAMF7, SDC1 and ICAM1). The gene discussed is LY9; the disease is alveolar rhabdomyosarcoma.