PAX5, LTB and CD44 (markers of stem cells) were significantly colocalized in the No. 7 cluster; SLAMF7, SDC1 (Syndecan-1) and ICAM1 were scattered among different clusters of rhabdomyosarcoma cells; while IL1A, IL5RA, IL7 and LY9 were not detected in rhabdomyosarcoma cells (Fig. 9b). The gene discussed is ICAM1; the disease is rhabdomyosarcoma.