In this study, we employed CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) editing technology to knock-in the EGFR C797S mutation into an NSCLC cell line harboring EGFR L858R/T790M to establish a cellular model for studying the biology and treatment strategy of acquired EGFR C797S mutations. Here, EGFR is linked to non-small cell lung carcinoma.