Using a droplet digital PCR-based MethyLight (ddMethyLight) assay, we assessed whether the number of methylated DNA alleles in cfDNA was proportional to the tumor size in xenografted mice and then compared the sensitivity and specificity for the detection of CRC with cfDNA samples between the newly identified markers and THBD and c9orf50. We found that the newly identified markers were not superior to THBD and c9orf50 for the detection of CRC with cfDNA samples but that a panel of five markers were superior to THBD and c9orf50. This evidence concerns the gene C9orf50 and neoplasm.