In order to assess whether the inhibition of glycolysis in vivo during infection with B. burgdorferi would affect the levels of bacteria in the heart (where macrophage infiltration is most evident) or their inflammatory status, we treated the infected animals with the PFKFB3 inhibitor, 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3-PO) [46]. This evidence concerns the gene PFKFB3 and infection.