After tamoxifen injection, EGFP+ Dclk1 lineage area started to expand and replaced tomato+ tumor areas, and the proportion of EGFP+ Dclk1 lineage area increased up to 32.1 ± 6.93% on day 14 from 0.014 ± 0.002% on day 0 (Figure 4G and H, Figure 4—source data 1). This evidence concerns the gene DCLK1 and neoplasm.