The results showed that the expression of ACLY was significantly associated with HCC metastasis (P = .025), but not gender, age, hepatitis B (HBV) viral status, tumour‐node‐metastases (TNM) staging, alpha‐fetoprotein (AFP), cirrhosis, main tumour size, multimode and portal vein tumour thrombus (PVTT) (Table 2). This evidence concerns the gene AFP and neoplasm.