qRT-PCR with 2 additional edge- and core-derived glioma sphere models (Edge- and Core-derived g1053 spheres and g0573 spheres) showed that both PLAGL1 and CD109 were higher in the core-derived, yet CD133 was up in the edge-derived, glioma spheres in vitro (Figure 2G). Here, PLAGL1 is linked to central nervous system cancer.