It was highly accumulated in kidneys with uptake values of 99.46 ± 17.28 ID%/g at 1 h p.i. and 44.44 ± 5.43 ID%/g at 4 h p.i. The clearance in the blood was slow with uptake values of 5.99 ± 0.44 ID%/g at 1 h p.i. and 4.16 ± 0.12 ID%/g at 4 h p.i. 22Rv1 tumor showed a slightly increased uptake of 6.83 ± 1.16 ID%/g at 4 h p.i. As with most PSMA probes, the uptake values of Al18F-PSMA-CM in kidneys (−79.9%, 74.76–14.99 ID%/g) and 22Rv1 tumor (−84.3%, 6.16–0.97 ID%/g) at 2 h p.i. were decreased when 50 μg of ZJ-43 was co-injected. The gene discussed is FOLH1; the disease is neoplasm.