qRT-PCR, Western Blot, and Immunofluorescence (IF) experiments showed that the mRNA and protein expression of ZEB1 in the group treated with si-circ-ZDHHC5 + miR-217 mimics was noticeably decreased compared with the group treated withsi-circ-ZDHHC5 in ESCC cell lines (Figures F–H and Supplementary Figure S1B). This evidence concerns the gene ZEB1 and esophageal squamous cell carcinoma.