PBRM1 and neoplasm: Next-generation sequencing (NGS) was performed on formalin-fixed, paraffin-embedded (FFPE) surgical tissue sections (ACT Genomics Co., Ltd.; mean depth, 1574+; uniformity 93%), which showed that the primary tumor had the A1209fs mutation of the PBRM1 gene and a low tumor mutational burden (TMB) (6.9 mut/Mb.