The results of RNA-sequence in our center indicated that six (CD109, CD96, FNI, HNMT, MMP9 and NFKBIZ) of the 14 OS-associated immune genes exhibited dysregulated expression between NMIBC and MIBC, indicating that dysregulated expression levels of these genes served an important role in the malignant progression of BLCA. The gene discussed is CD96; the disease is bladder transitional cell carcinoma.