FN1 and neoplasm: Compared to it, the U87MG cell line showed higher EDB-FN expression in 3D culture (p < 0.001, compared to 2D cultured U87MG, Welch's t test), which mimics the tumor microenvironment, and the highest EDB-FN expression when xenografted into animals (p < 0.0001, compared to 3D cultured U87MG, Welch's t test).