ACSS3 and prostate intraepithelial neoplasia: IHC and HE staining demonstrated that loss of ACSS3 increased the ratio of prostatic intraepithelial neoplasia (PIN, a pathological marker of a precancerous lesion) in the anterior prostates of transgenic mice (Figure 7B), suggesting that normal prostatic gland tissue was transforming into tumors due to the absence of ACSS3.