Using RNA-seq, we profiled the differential transcriptomes of primary HSCs associated with EZH2 inhibition, and mechanistically identified critical genes involved in liver fibrosis as novel direct targets under the epigenetic regulations of EZH2 and JMJD3, including the TGF-β1 pseudoreceptor BAMBI gene. This evidence concerns the gene EZH2 and Hepatic fibrosis.