According to the cryosection data on CTLs infiltration shown in Figure 2E, we chose one day after OT-I CTL transfer to take time-lapse intravital imaging of the liver metastasis microenvironment, in which CTLs expressed the tdTomato fluorescent protein (shown in blue) and B16 melanoma cells expressed the C3 probe (shown in red [alive] or green [apoptosis]). This evidence concerns the gene C3 and melanoma.