On day 9, adoptive transfer of OT-I CTLs inhibited the growth of B16-C3-OVA liver metastasis, with the mean percentage of the tumor area in the liver section only 0.03 ± 0.01%, which was much less than that of the B16-C3 + CTLs group, with 38.99 ± 11.8% (Figure 2C-D and S2C). Here, C3 is linked to neoplasm.