In order to further verify the role of Akt/mTOR and ERK in rhHMGB1-induced HGC-27 cell proliferation and migration, the effects of LY294002, rapamycin and U0126 on rhHMGB1-induced GC cell proliferation and migration were examined using EdU, colony formation, wound healing and transwell assays. This evidence concerns the gene MTOR and gastric cancer.