To further elucidate the molecular mechanisms by which knockdown of DLEU2 expression suppressed proliferation and invasion by CRC cells in vitro, we used qRT-PCR and western blotting to assess mRNA and protein levels of the proliferation markers cyclin‐dependent kinase 6 (CDK6) and cyclin-dependent kinase inhibitor 1A (CDKN1A), as well as the epithelial-mesenchymal transition (EMT) markers ZEB1 and ZEB2 in HT29 cells. This evidence concerns the gene DLEU2 and colorectal carcinoma.