First, we showed that the expression of CASC8 was significantly higher in tumors than in adjacent tissues using the TCGA database, which provides a rationale for the study of CASC8. The A549 and H460 NSCLC cell lines, which have relatively high levels of CASC8, and the H1975 NSCLC cell line, which harbors an EGFR T790M mutation, were selected as experimental models for this study, and a series of in vitro experiments were performed. Here, EGFR is linked to non-small cell lung carcinoma.