ALKAL1 and colorectal cancer: It is worth noting that ALKAL1 silencing is not associated with colorectal cancer RKO and SW480 cell proliferation (Figure 3C, 3D), plate colony formation (Figure 3E) and cell cycle distribution (Figure 3F) via CCK-8 assay, plate clone formation assay and cell cycle analysis with PI staining using Flow cytometry, respectively.