In order to further confirm whether ALKAL1 is expressed in colorectal cancer cells, ALKAL1 mRNA and protein expression were examined in selected 8 colorectal cancer cell lines (Caco-2, DLD-1, HCT-8, LS 174T, RKO, SW480, SW620 and T84), and the results showed that ALKAL1 is detectable in colorectal cancer epithelial cell lines (Figure 1G, 1H). This evidence concerns the gene ALKAL1 and colorectal cancer.