Vigil is an autologous tumor vaccine, produced from harvested tumor tissue and transfected with a plasmid that encodes the GM-CSF gene as well as a bifunctional short hairpin RNA (bi-shRNA) construct which targets furin as demonstrated by downstream knockdown of TGFβ1 and TGFβ2 58. The gene discussed is TGFB2; the disease is neoplasm.