LDLR and familial hyperaldosteronism: Sub-cloning of LDLR fragments was required, since the 3′-untranslated region of the mRNA contained Alu repeats, which had to be eliminated to identify sequences unique to LDLR. Southern hybridization experiments using samples from 50 patients with verified clinical diagnoses of FH revealed a 5 kb deletion, encompassing exons 4–6 of LDLR segregating in a family with FH (Mandelshtam et al., 1993).