But in Sirt1+/− mice on HFD, body weight, fat content and hepatic steatosis are increased, adipose tissue macrophage infiltration and expression of inflammatory genes, indicated by the expression of F4/80, TNF-α, IL-1, and IL-6, are enhanced in epididymal fat (Xu et al., 2010). This evidence concerns the gene SIRT1 and fatty liver disease.