The changes were strikingly reversed by administration of H2S. It has been demonstrated that the PI3K/Akt/mTOR cascade plays a role in the development of NAFLD and ROS elevation can lead to the activation of PI3K/AKT/mTOR cascade (Chen et al., 2017; Urasaki et al., 2018). The gene discussed is MTOR; the disease is metabolic dysfunction-associated steatotic liver disease.