As shown in Table 1, NETO2 overexpression was associated with ESCC Histological grade (P=0.005), TNM stage (P=0.003), Tumor size (P=0.024), depth of invasion (P<0.01) and lymph node metastasis (P=0.007) but we did not observe difference with patient's age and sex. This evidence concerns the gene NETO2 and esophageal squamous cell carcinoma.