CHAT and Alzheimer disease: For ChAT, there was no change in colocalisation with NNMT expression, with TOS (0.45 ± 0.044 vs. 0.42 ± 0.073, P = 0.86), M1 (63.7 ± 10.5% vs. 63.8 ± 5.2%, P = 0.99) and M2 (75 ± 4% vs. 72.4 ± 6.7%, P = 0.86) not significantly different in NDC and AD subjects (n = 5 and 3, respectively).