The heatmap of these seven immune-related genes expression level in GSE42352 indicated that five of these genes (CDCA7, GZMA, SLC7A7, VAMP8, and EVI2B) were highly expressed in the osteosarcoma group, but two of these genes (IFITM3 and ACTA2) were highly expressed in the normal group (Figure 5D). This evidence concerns the gene ACTA2 and osteosarcoma.