Western blotting analysis revealed that SAPCD2 downregulation increased the expression levels of phosphorylated MST1/2 (p-MST1/2), phosphorylated LATS1 (p-LATS1), and phosphorylated YAP1 (p-YAP1), and decreased the nuclear translocation of YAP and TAZ, which are downstream effectors of Hippo signaling; however, SAPCD2 downregulation had no effect on the total levels of MST1 and LATS1 in fibrosarcoma cells (Figure 5C). Here, YAP1 is linked to fibrosarcoma.