NKG2D-CAR T cells exhibited robust CD107a degranulation (Figure 4A) and intracellular production of TNF-α (Figure 4B) and IFN-γ (Figure 4C) when co-cultured with AML and T-ALL lines, but not in response to the murine melanoma cell line B16 (negative for human NKG2D-ligands). Here, TNF is linked to acute lymphoblastic leukemia.