EIF4EBP1 and infection: Then, we analyzed the phosphorylation status of 4E-BP1 and S6, as substrates of mTOR activity, and we observed a significant increase of 4E-BP1 and S6 phosphorylation after 2 hpi, which was not evident after 6 h of infection (Figure 5A), both facts supporting an early activation of mTOR.