In line with this, genetic variants in transmembrane 6 superfamily member 2 (TM6SF2) and patatin-like phospholipase domain-containing protein 3 (PNPLA3) genes, resulting in loss-of-function and decreased VLDL secretion and lipid droplet remodeling, respectively, were robustly linked to NAFLD development and its progression (50, 51). This evidence concerns the gene PNPLA3 and metabolic dysfunction-associated steatotic liver disease.