Additionally, Sirt-1 also plays a significant role in the glucose and fat homeostasis by regulating various transcription factors; reduction in Sirt-1 activity due to either HFD or unhealthy diet leads to insulin resistance (Chen et al., 2013), altered immunity, mitochondrial apoptosis, and NAFLD (Martins, 2017b). This evidence concerns the gene SIRT1 and metabolic dysfunction-associated steatotic liver disease.