Indeed, Sanger sequencing was able to resolve the polymorphisms in the CRC cell lines RKO, DLD-1, and VaCo-703 which are homozygous for all NAT2 polymorphisms, but not in HepG2 cells where heterozygosity was observed at all 7 polymorphic sites (Fig. 5A). The gene discussed is NAT2; the disease is colorectal carcinoma.