Vehicle and untreated day 7 UUO groups exhibited substantial renal fibrosis based upon an increase in the total kidney collagen content, as measured by the hydroxyproline assay (Figure 8A), an increase in the interstitial deposition of collagen IV (Figure 8B,C,E), and interstitial accumulation of α-SMA+ myofibroblasts (Figure 8F). The gene discussed is ACTA1; the disease is renal fibrosis.