The objectives of the current study were to determine the benefits of genistein supplement on NASH induced by a high-fat, high-fructose (HFHF) diet in the setting of estrogen deficiency and its effects on lipid metabolism, oxidative stress, hepatocyte apoptosis, hepatic PPARγ expression, and adiponectin levels. This evidence concerns the gene PPARG and metabolic dysfunction-associated steatohepatitis.